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Dave nλ=2dsinθGreat to see Rebekka Wild's group going from strength to strength.Structural basis for human chondroitin sulfate chain polymerization | bioRxiv<a href="https://xtaldave.net/tags/StructuralBiology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#StructuralBiology</a> <a href="https://xtaldave.net/tags/CryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEM</a> <a href="https://xtaldave.net/tags/Glycotime" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Glycotime</a> <a href="https://xtaldave.net/tags/Science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Science</a> <a href="https://xtaldave.net/tags/Research" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Research</a> <a href="https://www.biorxiv.org/content/10.1101/2025.03.21.644485v1?rss=1" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.biorxiv.org/content/10.1101/2025.03.21.644485v1?rss=1</a>

Ed EngHave you used one of the NIH sponsored <a href="https://fediscience.org/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a> centers and thought that was a great resource but looking to meet & interact with your peers? Ready to share and learn about other people’s experiences with using cryoEM?Then join us at the <a href="https://fediscience.org/tags/MM2025" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MM2025</a> Pre-Meeting Congress (PMCX61) on Transformative CryoEM in Salt Lake City, UT on Sunday, July 27, 2025.Early registration deadline is May 8, 2025:<a href="https://mmconference.microscopy.org/registration-information" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://mmconference.microscopy.org/registration-information</a>

PLOS BiologyBacterial ABC peptide transporters are involved in processes like nutrient uptake. This study solves <a href="https://fediscience.org/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a> structures of the <a href="https://fediscience.org/tags/Ecoli" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Ecoli</a> dipeptide <a href="https://fediscience.org/tags/transporter" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#transporter</a> DppABCDF in both apo & ATP analog-bound forms, providing insights into assembly & import mechanism <a href="https://fediscience.org/tags/plosbiology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#plosbiology</a> <a href="https://plos.io/3DxP8pU" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://plos.io/3DxP8pU</a>

Guillaume Gaullier ❄️🔬New preprint I contributed to: <a href="https://doi.org/10.1101/2025.02.17.638584" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://doi.org/10.1101/2025.02.17.638584</a>I helped this group do atomic model building and refinement in their <a href="https://fediscience.org/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a> maps.This was more model building than I had done with all my previous projects combined! And it made me streamline the process I described in this thread: <a href="https://fediscience.org/@Guillawme/113281867808537736" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://fediscience.org/@Guillawme/113281867808537736</a>

CellBioNews<a href="https://scientificnetwork.de/tags/Bacterial" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Bacterial</a> <a href="https://scientificnetwork.de/tags/flagellar" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#flagellar</a> motors: Study sheds light on their <a href="https://scientificnetwork.de/tags/ion" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#ion</a>-driven mechanisms.<a href="https://scientificnetwork.de/tags/CryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEM</a> <a href="https://scientificnetwork.de/tags/sodium" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#sodium</a> <a href="https://scientificnetwork.de/tags/vibrio" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#vibrio</a> <a href="https://phys.org/news/2025-02-bacterial-flagellar-motors-ion-driven.html" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://phys.org/news/2025-02-bacterial-flagellar-motors-ion-driven.html</a>

Pereda Lab<a href="https://mstdn.science/tags/course_announcement" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#course_announcement</a> Colleagues of the Department of Crystallography and Structural Biology of the Institute of Physical-Chemistry "Blas Cabrera", just announced a new edition of their course:Macromolecular Crystallography & Cryo-EM School - MCCS-2025Registration is now open 🔗 <a href="https://www.xtal.iqf.csic.es/MCCS2025/" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.xtal.iqf.csic.es/MCCS2025/</a> 📅 12-16 May 2025 📍 Institute of Physical-Chemistry "Blas Cabrera" CSIC, Madrid, SpainHighly recommended <a href="https://mstdn.science/tags/Structural_biology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Structural_biology</a> <a href="https://mstdn.science/tags/Macromolecular_Crystallography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Macromolecular_Crystallography</a> <a href="https://mstdn.science/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a> <a href="https://mstdn.science/tags/CSIC" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CSIC</a>

StructBiolCommunicationsCryoCrane, a visualization tool for cryo-EM screening data, aims to provide an intuitive way to visualize micrographs and to speed up data analysis. <a href="https://mstdn.science/tags/CryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEM</a> <a href="https://mstdn.science/tags/SampleOptimization" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#SampleOptimization</a> <a href="https://mstdn.science/tags/GridScreening" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#GridScreening</a> <a href="https://doi.org/10.1107/S2053230X25000081" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://doi.org/10.1107/S2053230X25000081</a>

Guillaume Gaullier ❄️🔬Dealing with symmetry in single-particle <a href="https://fediscience.org/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a> data analysis can be super interesting, but can also turn into a massive headache... 😵‍💫

Guillaume Gaullier ❄️🔬The micrograph denoiser in CryoSPARC is really good! Fast training and denoising, excellent output (more helpful than topaz denoise; both run with their default settings).Why didn't I start using it earlier??<a href="https://fediscience.org/tags/CryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEM</a>

eLifeCryogenic Electron Microscopy: MagIC beads for scarce macromolecules. <a href="https://fediscience.org/tags/CryoEm" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEm</a> <a href="https://elifesciences.org/articles/105335?utm_source=mastodon&utm_medium=social&utm_campaign=organic_insights" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://elifesciences.org/articles/105335?utm_source=mastodon&utm_medium=social&utm_campaign=organic_insights</a>

Guillaume Gaullier ❄️🔬During the fall semester, I taught a few hours in a course called "Introduction to <a href="https://fediscience.org/tags/biochemistry" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biochemistry</a>", taken by master students in a <a href="https://fediscience.org/tags/Biophysics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Biophysics</a> program. This course is for students with a bachelor in physics, to get them up to speed with biochemistry. It's really tough, they have to absorb in one semester the equivalent of a bachelor in biochemistry.I gave a lecture on methods to determine the 3D structure of macromolecules. The exam question on this part of the course is pretty gentle (because it's impossible to go in details in one lecture): list the methods seen in class, give a very broad overview of each, give one strength and one weakness for each.For <a href="https://fediscience.org/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a>, both students answered "it's expensive" as a weakness. 😂 Honestly it's a passing answer, it's so true. But funny that this is the one that came to mind first.They also seem quite confused about <a href="https://fediscience.org/tags/NMR" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#NMR</a>.So I think these exam results are telling a lot more about the teacher than the students. 🙃

Guillaume Gaullier ❄️🔬It's great to *finally* have both a direct work-related reason and enough free time these past two days to immerse myself in the two atomic resolution <a href="https://fediscience.org/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a> papers from 2020 and give them a good, thorough, cover-to-cover read. 📖 🤓 ❄️ 🔬Feeling like it's an important step through the never-ending backlog of important papers in this field. 😌Nakane et al. 2020: <a href="https://doi.org/10.1038/s41586-020-2829-0" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://doi.org/10.1038/s41586-020-2829-0</a>Yip et al. 2020: <a href="https://doi.org/10.1038/s41586-020-2833-4" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://doi.org/10.1038/s41586-020-2833-4</a>

StructBiolCommunicationsCryoCrane is a visualization tool for cryo-EM screening data that aims to provide an intuitive way to visualize micrographs and to speed up data analysis. <a href="https://mstdn.science/tags/CryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEM</a> <a href="https://mstdn.science/tags/SampleOptimization" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#SampleOptimization</a> <a href="https://mstdn.science/tags/GridScreening" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#GridScreening</a> <a href="https://doi.org/10.1107/S2053230X25000081" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://doi.org/10.1107/S2053230X25000081</a>

Stephen RoyleThis is pretty amazing. TMEM206 structure determined using a single HEK cell colony!"MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony”<a href="https://www.biorxiv.org/content/10.1101/2025.01.10.632437v1" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.biorxiv.org/content/10.1101/2025.01.10.632437v1</a><a href="https://biologists.social/tags/StructuralBiology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#StructuralBiology</a> <a href="https://biologists.social/tags/CryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEM</a>

Guillaume Gaullier ❄️🔬Model building in high-resolution <a href="https://fediscience.org/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a> maps is more fun when it's a protein purified from its native source and nobody knows the correct sequence. 😅 <a href="https://fediscience.org/tags/ModelAngelo" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#ModelAngelo</a> to the rescue!

Dave nλ=2dsinθNEW - From us!Capture, mutual inhibition and release mechanism for aPKC–Par6 and its multisite polarity substrate Lgl <a href="https://www.nature.com/articles/s41594-024-01425-0" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.nature.com/articles/s41594-024-01425-0</a>It's be a long and difficult birth, but it is finally out there. Lovely bit of integrative CryoEM revealing a crucial kinase/substrate complex in the determination of cell polarity - how cells know which way is up. <a href="https://xtaldave.net/tags/StructuralBiology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#StructuralBiology</a> <a href="https://xtaldave.net/tags/CryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEM</a> <a href="https://xtaldave.net/tags/MX" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MX</a> <a href="https://xtaldave.net/tags/Crystallography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Crystallography</a> <a href="https://a.gup.pe/u/strucbio" class="u-url mention" rel="nofollow noopener noreferrer" target="_blank">@strucbio</a>

mechanochemistryKicking off 2025 with a new paper by Ferdos Abid Ali & Alex Zwetsloot et al.KIF1C activates and extends dynein movement through the FHF cargo adapter. Nat Struct Mol Biol (2025).<a href="https://doi.org/10.1038/s41594-024-01418-z" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://doi.org/10.1038/s41594-024-01418-z</a><a href="https://mstdn.science/tags/cytoskeleton" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cytoskeleton</a> <a href="https://mstdn.science/tags/biophysics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biophysics</a> <a href="https://mstdn.science/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a> <a href="https://mstdn.science/tags/MotorHeads" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MotorHeads</a> <a href="https://mstdn.science/tags/CellBiology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CellBiology</a>

jobRxivPostdoctoral Scholar-Structural Biology in Protein Dynamics Icahn School of Medicine at Mount SinaiJoin our lab as a <a href="https://mas.to/tags/StructuralBiology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#StructuralBiology</a> <a href="https://mas.to/tags/Postdoc" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Postdoc</a> to study proteins in <a href="https://mas.to/tags/Alzheimers" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Alzheimers</a> & <a href="https://mas.to/tags/Cancer" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Cancer</a> using <a href="https://mas.to/tags/CryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEM</a> & <a href="https://mas.to/tags/XrayCrystallography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#XrayCrystallography</a> at Mount Sinai!See the full job description on jobRxiv: <a href="https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai</a>... <a href="https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-postdoctoral-scholar-structural-biology-in-protein-dynamics-2/?feed_id=89569" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-postdoctoral-scholar-structural-biology-in-protein-dynamics-2/?feed_id=89569</a>

Dave nλ=2dsinθStructural mechanism of CB1R binding to peripheral and biased inverse agonists | Nature CommunicationsLovely bit of work. <a href="https://xtaldave.net/tags/CryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoEM</a> <a href="https://xtaldave.net/tags/Crystallography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Crystallography</a> <a href="https://xtaldave.net/tags/Science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Science</a> <a href="https://xtaldave.net/tags/Research" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Research</a> <a href="https://a.gup.pe/u/strucbio" class="u-url mention" rel="nofollow noopener noreferrer" target="_blank">@strucbio</a> <a href="https://www.nature.com/articles/s41467-024-54206-0" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.nature.com/articles/s41467-024-54206-0</a>

Guillaume Gaullier ❄️🔬<a href="https://fediscience.org/@posertinlab" class="u-url mention" rel="nofollow noopener noreferrer" target="_blank">@posertinlab</a> I have used <a href="https://fediscience.org/tags/CryoSPARC" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CryoSPARC</a>'s tree view since forever because it's a lot easier to understand the relationships between jobs. Even when it was slow on large projects, I put up with it because this view is so helpful. Back when I learned single-particle <a href="https://fediscience.org/tags/cryoEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cryoEM</a>, with <a href="https://fediscience.org/tags/RELION" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#RELION</a>, I used to draw the job graph by hand as I set up new jobs, so I could remain oriented within large projects.The performance of the tree view in CryoSPARC 4.6 is really excellent, it's a big improvement in usability compared to previous versions. Now the only thing that could make the tree view better is an export function as I described here: <a href="https://discuss.cryosparc.com/t/job-graph-figure/15228" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://discuss.cryosparc.com/t/job-graph-figure/15228</a>

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