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#force

26 posts18 participants1 post today
Rxiv mechanobio

📰 "A Fibroblast State Choreographs an Epithelial YAP-dependent Regenerative Program Essential to (Pre)malignancy via ECM-mediated Mechanotransduction"
biorxiv.org/content/10.1101/20 #Mechanotransduction #Force #Cell #Ecm

bioRxiv · A Fibroblast State Choreographs an Epithelial YAP-dependent Regenerative Program Essential to (Pre)malignancy via ECM-mediated MechanotransductionChronic lung injury generates metaplasia which occasionally, but ominously, progresses to squamous dysplasia and squamous lung cancer. To identify mechanisms through which disrupted tissue homeostasis contributes to malignant initiation and progression, we used in vivo and in vitro heterotypic recombinant models of human bronchial epithelial cells (hBECs) and fibroblasts. We demonstrate that injury-associated TGF-β signaling creates a fibroblast state dependent upon HSP47 upregulation. These fibroblasts accumulated collagen, thus elevating tissue stiffness and activating mechanosignaling that sustained YAP-dependent embryonic-like, pro-malignant activities in adjacent hBECs. This Stress/Tension-Instructive Fibroblast (STIF) state, exhibited by stressed fibroblasts in premalignant and malignant lesions across multiple cancer types, was sufficient to reprogram disease-free hBECs to metaplasia and to drive hBECs with compromised tumor suppressor function to dysplasia, yet could be inhibited and reversed. STIFs suffice to activate epithelial phenotypes reminiscent of oncogene-mediated cell transformation and induce (pre)malignancy via increased force transmission, providing novel targets for prevention. ### Competing Interest Statement The authors have declared no competing interest. Cancer Research UK, 27145, 29071, 29078, 29068 National Cancer Institute, https://ror.org/040gcmg81, 5R35CA197694, 5R50CA211543
Rxiv mechanobio

📰 "Is Language a Mechanical Signal? Cytoskeletal Responses to Speech in Yeast"
biorxiv.org/content/10.1101/20 #Cytoskeletal #Cytoskeleton #Mechanical #Force

bioRxiv · Is Language a Mechanical Signal? Cytoskeletal Responses to Speech in YeastWhat if language is not only a medium for human connection but a vibrational force that leaves structural traces in living cells? This study explores how audible sound, especially structured components of human speech, affects the cytoskeleton of Saccharomyces cerevisiae. Using a direct-contact acoustic system, we exposed yeast to distinct sound types: tonal vibrations, broadband noise, and consonant phonemes. Fluorescence microscopy revealed that tonal stimuli with coherent low-frequency patterns enhanced actin polymerization and shmoo formation, both markers of polarity and mating. In contrast, broadband noise disrupted actin integrity, while consonants produced no measurable effects. These results suggest that rhythmic continuity and spectral coherence, key features of speech, can modulate cytoskeletal organization in non-auditory cells. By reframing language as mechanical input rather than semantic content, this study bridges microbial cell biology with acoustic ecology and proposes a new lens for exploring how human soundscapes physically influence living systems. ### Competing Interest Statement The authors have declared no competing interest.
Rxiv mechanobio

📰 "Label-free microscope for rheological imaging of cells"
arxiv.org/abs/2507.07470 #Physics.Optics #Physics.Bio-Ph #Extracellular #Force

arXiv logo
arXiv.orgLabel-free microscope for rheological imaging of cellsMany essential cellular functions depend on the viscoelastic properties of the cytoplasm. While techniques such as optical tweezers and atomic force microscopy can measure these properties, their reliance on localized probes prevents intracellular imaging and perturbs native cellular behaviour. Label-free microscopy offers a non-invasive alternative for observing intracellular dynamics. However, limitations in signal-to-noise ratio and imaging speed typically restrict analysis to diffusivity, leaving cellular viscous properties inaccessible. Here, we introduce rheoSCAT, a label-free, phase-sensitive microscope engineered with ultra-low phase noise. This system enables measurements of intracellular dynamics at frequencies up to 50 kHz, twenty times faster than previous label-free approaches. Applied to live cancer cells, this technique establishes a connection between label-free microscopy and rheology. The high speed of our technique reveals viscoelastic behaviours that were previously inaccessible, which we show are consistent with probe-based microrheology observations. The rheological images produced distinguish intra- and extracellular regions with high contrast, resolve spatial variations in cellular mechanics, and enable monitoring of cellular state and stress over time. The ability to quantitatively map intracellular energetics and viscoelasticity offers a powerful tool for advancing fundamental cell biology, cancer research, clinical diagnostics, and drug development.
Rxiv mechanobio

📰 "Lipid composition effects on the number and size of liposomes formed by the inverted emulsion method"
biorxiv.org/content/10.1101/20 #Force #Cell

bioRxiv · Lipid composition effects on the number and size of liposomes formed by the inverted emulsion methodLiposomes have been widely employed as membrane scaffolds in the construction of minimal cell models. In 2002, the inverted emulsion method was introduced as a novel technique for generating giant liposomes by transferring water-in-oil droplets across an oil/water interface. This technique enables the encapsulation of purified proteins or cytoplasmic extracts into cell-sized liposomes under physiological buffer conditions, and has since become a cornerstone in bottom-up synthetic biology. Despite its broad application over the past two decades, the effects of lipid composition on the production yield and size distribution of liposomes generated by the inverted emulsion method remain largely unknown. In this study, we systematically investigated the effects of phospholipid composition on the production yield and size distribution of liposomes generated using the inverted emulsion method. We used a natural phosphatidylcholine purified from chicken egg yolk (egg PC) as the base membrane component, and examined the extent to which substituting a fraction of egg PC with other phospholipids, differing in net electric charge and/or the molecular weight of their hydrophilic head groups, affects the number and size distribution of liposomes. We found that a 10% replacement of egg PC with charged phospholipids significantly enhances the production yield of liposomes by approximately tenfold, transformed by both the natural gravitational force and the greater force applied by centrifugation. In addition, lipids with smaller head groups slightly enhance the production yield in both methods. Lipids with larger head groups also slightly enhance the production yield and promote the formation of larger liposomes in the natural sedimentation method, but these effects are diminished in the centrifugal sedimentation method, presumably due to the strong centrifugal force. These findings provide valuable guidelines for optimizing preparation protocols for minimal cell models. ### Competing Interest Statement The authors have declared no competing interest.
Rxiv mechanobio

📰 "Indenting multi-cellular spheroids with various cantilever tip geometry"
biorxiv.org/content/10.1101/20 #Force #Cell

bioRxiv · Indenting multi-cellular spheroids with various cantilever tip geometrySpheroids are of great interest in the study of cancer as they can partially mimic the tumour microenvironment, thus allowing to investigate several aspects of cell - microenvironment interactions in healthy and diseased conditions, including those pertaining to mechanobiology. Atomic Force Microscopy (AFM) is a versatile tool for studying biological samples and their mechanobiological properties. In AFM, the tip shape and dimensions determine the contact geometry between the tip and the sample and the length scales at which the mechanical properties are probed. Given the complex multiscale structure of spheroids, the choice of tip geometry and size would allow, in principle, to dissect the mechanical response of the overall system into the contributions of the constituents, from the single cell level to the cellular aggregate. In this work, we studied the mechanical properties of spheroids derived from four cell lines (A549, NHLF, HT-29, CCD-18Co). Our studies revealed that using different contact geometries in the fitting procedure results in significantly different Young's modulus values, highlighting the multiscale response of these complex cellular systems and the importance of a precise experiment design and choice of the AFM probe for the nano-mechanical measurements. We observed that the location of F-actin filaments is correlated to the rigidity of the spheroids. ### Competing Interest Statement The authors have declared no competing interest. European Union’s Horizon 2020 Marie Skłodowska-Curie Action, 812772
Rxiv mechanobio

📰 "A new approach for high-content traction force microscopy to characterize large cell ensembles"
biorxiv.org/content/10.1101/20 #Forces #Force #Cell

bioRxiv · A new approach for high-content traction force microscopy to characterize large cell ensemblesAccurate measurements of cellular forces are important for understanding a wide range of biological processes where traction plays a major role. The characterization of mechanical properties is needed to unravel complex phenomena like migration, morphogenesis, mechanotransduction, or shape regulation, but accurate data on large numbers of single cells remain scarce and challenging. The capacity to measure forces in populations of cells and to identify subsets within heterogeneous ensembles would enable to reveal and manipulate their intrinsic complexity. Traction Force Microscopy (TFM) is a technology that can quantify the contractile forces exerted by cells via measuring the displacement of fluorescent beads embedded on the surface of a soft substrate with precisely defined mechanical properties. However, conventional numerical approaches for measuring cellular forces using TFM are labor-intensive and often yield significant artifacts, making them ill-suited for high-throughput analysis. In this work, we propose using the Demons algorithm instead, leading to significant improvements in both computational efficiency and accuracy. Based computer simulations, we show that this methodology outperforms conventional approaches in terms of speed, it is less sensitive to the blur induced by out-of-focus images and improves the accuracy of force field reconstructions. Additionally, we conducted experiments using various cell lines and gels of distinct stiffness to demonstrate that the Demons algorithm is capable of real-time and effective at clustering cells according to their mechanotype. ### Competing Interest Statement The authors have declared no competing interest.
サファイア・ネオ

戦争、その正体とは・・・・・
戦争、勝者も敗者も無い、
สงครามเป็นอาวุธที่อันตรายที่สุดที่ชาติสามารถครอบครองได้

note.com/poison_raika/n/n7fd73

<>

note（ノート）戦争、その正体とは・・・・・｜ポイズン雷花「凶器」 戦争とは、国家が持ち得る最大の凶器。 盾でも刃でも無い。武器でも防具でも無い。 正しい呼び名は凶器で有る！ "อาวุธร้ายแรง" สงครามเป็นอาวุธที่อันตรายที่สุดที่ชาติสามารถครอบครองได้ ไม่มีทั้งโล่หรือใบมีด ทั้งอาวุธและ ชุดเกราะ ชื่อที่ถูกต้องคืออาวุธร้ายแรง! 「人災」 欲望、野心。それらが齎らす最大の人災 人々はそれをこう呼ぶ「戦争」 破壊と殺戮に基づく力の行使 憎しみを振り撒き 流血を促す悪しき心 "ภัยพิบัติจากฝ
#dialogue#name#force
United States

Commentary: Trump priorities clear: Derail medical and scientific research, invade MacArthur Park

The nation’s priorities are now crystal clear. We are adding ICE and Border Patrol agents, activating troop…
#UnitedStates #US #USA #america #americanneighborhood #child #country #criminalrecord #federalagent #force #homedepots #ice #Immigration #l.a. #lastyear #macarthurpark #nation #presidenttrump #trumppriority #unitedstatesofamerica #USnews #USTopics #USAnews
europesays.com/2227523/

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